Chapter 1: The Biotechnology Industry

What Is Biotechnology?

Who Uses Biotechnology?

Biotechnology Industry and Research

Governmental Regulation of Biotechnology

Industry Practices

Careers in Biotechnology

Chapter 1 Essay Questions

Additional Resources

Chapter 1 Vignettes

Bioethics Biotechnology: Good or Bad?

Biotech In The Real World: Bio-Rad: Then and Now

How To... Write an SOP

Careers In Biotech:Annabelle Tinoco, Quality Assurance Manager

Chapter 2: Laboratory Skills

Laboratory Safety

Laboratory Notebooks

Laboratory Equipment

Numerical Data

Significant Figures

Scientific Notation

Units of Measure

Preparing Solutions

Percent Solutions

Using Stock Solutions Given in Terms of “x”

Molar Solutions

Chapter 2 Essay Questions

Additional Resources

Chapter 2 Laboratory Activities

Activity 2.1 DNA Extraction and Precipitation

Activity 2.2 Pipetting

Activity 2.3 Kool-Aid Column Chromatography

Activity 2.4 Making Solutions

Activity 2.5 Titration

Activity 2.6 Writing a Standard Operating Procedure

Chapter 2 Extension Activities

Chapter 2 Vignettes

Bioethics: Waste Disposal

Biotech In The Real World: Who Invented Claritin? How Laboratory Notebooks Played Their Part

Careers In Biotech:Katie Dalpozzo, Laboratory Research Associate

How To... Use an Adjustable-Volume Micropipet

Chapter 3: Microbiology and Cell Culture

Microbiology and Cell Biology

Three Domains of Life

Microorganisms and History

Bacteria

Uses of Bacteria in Biotechnology

Culturing Bacteria in the Laboratory

Microbiological Techniques

Eukaryotic Cells

Uses of Eukaryotic Cells in Biotechnology

Eukaryotic Cell Culture

Chapter 3 Essay Questions

Additional Resources

Chapter 3 Laboratory Activities

Activity 3.1 Making Microbiology Media

Activity 3.2 Disk Diffusion Test （Modified Kirby-Bauer Test）

Activity 3.3 Microbes and Health:An Illustration of Koch's Postulates

Activity 3.4 Gram Staining

Activity 3.5 Quantifying Bacterial Numbers

Activity 3.6 Staining Eukaryotic Cells

Chapter 3 Extension Activities

Chapter 3 Vignettes

How To... Use Aseptic Technique to Transfer Bacteria

Careers In Biotech:Mihaela Sabau, Clinical Laboratory Scientist

Bioethics:Should Human Embryos Be Used for Research?

Biotech In The Real World: Artificial Life!

Chapter 4: DNA Structure and Analysis

Molecular Biology

DNA Structure

Recombinant DNA Technology

Restriction Enzymes

Ligases

Horizontal Agarose Gel Electrophoresis

Restriction Enzymes as Forensic Tools

Restriction Fragment Length Polymorphisms

Chapter 4 Essay Questions

Additional Resources

Chapter 4 Laboratory Activities

Activity 4.1 Restriction Site Prediction Using NEBcutter

Activity 4.2 Casting Agarose Gels

Activity 4.3 Dye Electrophoresis

Activity 4.4 Restriction Digestion and Analysis of Lambda DNA

Activity 4.5 Forensic DNA Fingerprinting

Activity 4.6 Plasmid Mapping

Chapter 4 Extension Activities

Chapter 4 Vignettes

Bioethics: Personal Genetic Information

Careers In Biotech: Charles Lo, Commercial Rotation Development Program Associate

How To... Set Up a Restriction Digest

Biotech In The Real World: Did the Baker Do It?

Chapter 5: Bacterial Transformation and Plasmid Purification

History of Bacterial Transformation and Plasmids

Plasmids: Structure and Function

Using Plasmids in Biotechnology

Transcriptional Regulation of Plasmids

Transforming Cells

Selection of Transformed Cells

Plasmid Purification

DNA Quantitation

Chapter 5 Essay Questions

Additional Resources

Chapter 5 Laboratory Activities

Activity 5.1 Bacterial Transformation with S3 Plasmid

Activity 5.2 Bacterial Transformation withpGLO Plasmid

Activity 5.3 Purification of S3 and pGLO Plasmids

Activity 5.4 DNA Quantitation

Chapter 5 Extension Activities

Chapter 5 Vignettes

Bioethics: A World without Antibiotics?

How To... Transform E. coli Using Electroporation

Biotech In The Real World:Plasmids and the Search for Energy

How To... Use a Microcentrifuge

Careers In Biotech: Denise Gangadharan,Acting Associate Director for Science

Chapter 6: The Polymerase Chain Reaction

Invention of PCR

What Is PCR?

PCR and DNA Replication

Thermal Cyclers

Types of PCR

PCR Optimization

Techniques Based on PCR

Real-World Applications of PCR

Chapter 6 Essay Questions

Additional Resources

Chapter 6 Laboratory Activities

Activity 6.1 STR PCR Analysis

Activity 6.2 GMO Detection by PCR

Activity 6.3 Detection of the Human PV92 Alu Insertion

Chapter 6 Extension Activities

Chapter 6 Vignettes

Careers In Biotech: Stan Hitomi,Math and Science Coordinator and Principal

How To... Program a Thermal Cycler

Biotech In The Real World: Biotech on the Pharm

Bioethics: Forensic DNA Databases—Is Your Privacy Protected?

Chapter 7: Protein Structure and Analysis

Protein Synthesis

Protein Structure

Proteins in Biology

Proteins in Biotechnology

Methods of Protein Analysis

Protein Quantitation

Protein Electrophoresis

Protein Chromatography

Bioinformatics

Chapter 7 Essay Questions

Additional Resources

Chapter 7 Laboratory Activities

Activity 7.1 Protein Quantitation Using the Bradford Assay

Activity 7.2 Size Exclusion Chromatography

Activity 7.3 GFP Purification by Hydrophobic Interaction Chromatography

Activity 7.4 SDS-PAGE of Fish Muscle

Activity 7.5 Biofuel Enzyme Assay

Activity 7.6 Exploring Bioinformatics with GFP

Chapter 7 Extension Activities

Chapter 7 Vignettes

Bioethics: Food or Fuel?

Careers In Biotech: Scott Chilton, Graduate Student

How To... Set Up a Vertical Electrophoresis System

Biotech In The Real World: Vaccines in Bananas

Chapter 8: Immunological Applications

The Immune System

Antibodies

Antibodies as Tools

Immunoassays

Enzyme-Linked Immunosorbent Assay （ELISA）

Western Blotting

Chapter 8 Essay Questions

Additional Resources

Chapter 8 Laboratory Activities

Activity 8.1 Ouchterlony Double Immunodiffusion Assay

Activity 8.2 Serum Antibody Detection by ELISA

Activity 8.3 Quantitative ELISA

Activity 8.4 Western Blotting

Chapter 8 Extension Activities

Chapter 8 Vignettes

How To... Select an Antibody

Bioethics: Treatment of HIV Positive People in the Developing World

Biotech In The Real World:Using FACS to Diagnose and Treat Cancer

Careers In Biotech:Amanda Williams, Clinical Lab Technician

Chapter 9: Research Projects

What Is Research?

Student Research Projects

Choosing a Research Project

Planning a Project

Experimental Design

Data Analysis

Communicating Research

Project Ideas

Research Tools and Techniques

Agricultural and Animal Research

Food Science

Humans and Health

Forensics

Energy and the Environment

Considerations and Tips for All Laboratory Projects

Chapter 9 Essay Questions

Additional Resources

Chapter 9 Vignettes

Careers In Biotech: Sophy Wong, HIV Specialist

Bioethics: The Case of Woo Suk Hwang

Biotech In The Real World: Abraham Lincoln High School Takes On the iGEM Challenge

How To... Read a Journal Article

Appendices

Appendix A: Fast Gel Protocol

Appendix B: Alternative Staining Methods for Agarose Gels

Appendix C:Using the SmartSpec Plus Spectrophotometer

Appendix D: Glossary

Appendix E: Laboratory Skills Assessment Rubric

Appendix F: Laboratory Notebook and Project Rubrics

Laboratory Notebook Rubric

Project Progress Rubric

Project Proposal Rubric

Presentation Content Rubric

Presentation Style Rubric

Appendix G: Trademarks and Legal Notices