Section A - Cells and macromloecules

A1 Cellular Classification

A2 Subcellular organelles

A3 Macromolecules

Abbreviations

F4 Recombimation

A4 Large macromolecular assemblies

Preface to the second edition

Preface to the first edition

Section B - Protein structure

B1 Amino acids

B2 Protein structure and function

B3 Protein analysis

C1 Nucleic acid structure

Section C - Properties of nucleic acids

C2 Chemical and physical properties of nucleic acids

C3 Spectroscopic and thermal properties of nucleic acids

C4 DNA supercoiling

Section D - Prokaryotic and eukaryotic chromosome structure

D1 Prokaryotic chromosome structure

D2 Chromatin structure

D3 Eukaryotic chromosome structure

D4 Genome complexity

D5 The flow of gengtic information

E1 DNA replication：an overview

Section E - DNA replication

E2 Bacterial DNA replication

E3 The cell cycle

E4 Eukaryotic DNA replication

Section F- DNA damage,repair and recombimation

F1 Mutagenesis

F2 DAN damage

F3 DAN repair

Section G - Gene manipulation

G1 DAN cloning：an overview

G2 Preparation of plasmid DNA

G3 Restriction dnzymes and electriphoresis

G4 Ligation.transformation and analysis of recombinants

Section H - Cloning vectors

H1 Desing of plasmid vectors

H2 Bacteriophage vectors

H3 Cosmide,YACs and BACs

H4 Eukaryotic vectors

Section I - Gene libraries and screening

I1 Genomic libraries

I2 cDNA libraries

I3 Screening procedures

Section J - Analysis and uses of cloned DNA

J1 Charactrization of clones

J2 Nucleic acid sequencing

J3 Polymeraes chain reaction

J4 Organization of clonen genes

J5 Mutagenesis of cloned genes

J6 Applications of cloning

Section K - Transcription in prokaryotes

K1 Basic principles of transcription

K2 Escherichia coli RNA poly merase

K3 The E. coli a 70 promoter

K4 Transcription,initiation,elongation and etrmination

Section L - Regulation of transcrlption in prokaryotes

L1 The lac operon

L2 The trp operon

L3 Transcriptional regulation by alternative or factors

Section M - Transcription in eukaryotes

M1 The three RNA polymerases:characterization and function

M2 RNA polⅠ genes:the ribosomal repeat

M3 RNA pol Ⅲ genes： 5S and tRNA transcription

M4 RAN PolⅡgenes;promoters and enhancers

M5 General transcripton factors and RNA Pol Ⅱinitiation

Section N - Regulation of transcription in eukaryotes

N1 Eukaryotic transcription facrots

N2 Examples of transcriptional regulation

Section O - RNA processing and RNPs

O1 rRNA processing and ribosornes

O2 tRNA processing,RNase P and ribozymes

O3 mRNA processing, hnRNPs and snRNPs

O4 Alternative mRNA processing

Section P - The genetic code and tRNA

P1 The genetic code

P2 tRNA structuer and function

Section Q - Protenin symthesis

Q1 Aspects of protein symthesis

Q2 Mechanism of protein symthesis

Q3 Initiation in eukaryotes

Q4 Translational control and post-translational events

R1 Introduction to viruese

Section R - Baclexiophages and eukaryotic viruses

R2 Bacterophages

R3 DNA viruses

R4 RNA viruses

Section S - Tumor viruses and oncogenes

S1 Oncogenes found in turnor viruses

S2 Categories of on cogenes

S3 Tumor suppressor genes

S4 Apoptosis

Futrher reading

Multiple choice questions

Ansers

Index