Section A-Cells and macromolecules

A1 Cellular classification

A2 Subcellular organelles

A3 Macromolecules

A4 Large macromolecular assemblies

Section B-Protein structure

B1 Amino acids

B2 Protein structure and function

B3 Protein analysis

Section C-Properties of nucleic acids

C1 Nucleic acid structure

C2 Chemical and physical properties of nucleic acids

C3 Spectroscopic and thermal properties of nucleic acids

C4 DNA supercoiling

Section D-Prokaryotic and eukaryotic chromosome structure

D1 Prokaryotic chromosome structure

D2 Chromatin structure

D3 Eukaryotic chromosome structure

D4 Genome complexity

Section E-DNA replication

E1 DNA replication:an overview

E2 Bacterial DNA replication

E3 Eukaryotic DNA replication

Section F-DNA damage,repair and recombination

F1 Mutagenesis

F2 DNA damage

F3 DNA repair

F4 Recombination

Section G-Gene manipulation

G1 DNA cloning:an overview

G2 Preparation of plasmid DNA

G3 Restriction enzymes and electrophoresis

G4 Ligation,transformation and analysis of recombinants

Section H-Cloning vectors

H1 Design of plasmid vectors

H2 Bacteriophage vectors

H3 Cosmids and YACs

H4 Eukaryotic vectors

Section I-Gene libraries and screening

I1 Genomic libraries

I2 cDNA libraries

I3 Screening procedures

Section J-Analysis and uses of cloned DNA

J1 Characterization of clones

J2 Nucleic acid sequencing

J3 Polymerase chain reaction

J4 Organization of cloned genes

J5 Mutagenesis of cloned genes

J6 Applications of cloning

Section K-Transcription in prokaryotes

K1 Basic principles of transcription

K2 Escherichia coli RNA polymerase

K3 The E.coliσ70 promoter

K4 Transcription initiation,elongation and termination

Section L-Regulation of transcription in prokaryotes

L1 The lac operon

L2 The trp operon

L3 Transcriptional regulation by alternativeσfactors

Section M-Transcription in eukaryotes

M1 The three RNA polymerases:characterizationand function

M2 RNA Pol Ⅰgenes:the ribosomal repeat

M3 RNA Pol Ⅲ genes:5S and tRNA transcription

M4 RNA Pol Ⅱ genes:promoters and enhancers

M5 General transcription factors and RNA Pol Ⅱ initiation

Section N-Regulation of transcription in eukaryotes

N1 Eukaryotic transcription factors

N2 Examples of transcriptional regulation

Section O-RNA processing and RNPs

O1 rRNA processing and ribosomes

O2 tRNA processing,RNase P and ribozymes

O3 mRNA processing,hnRNPs and snRNPs

O4 Alternative mRNA processing

Section P-The genetic code and tRNA

P1 The genetic code

P2 tRNA structure and function

Section Q-Protein synthesis

Q1 Aspects of protein synthesis

Q2 Mechanism of protein synthesis

Q3 Initiation in eukaryotes

Q4 Translational control and post-translational events

Section R-Bacteriophages and eukaryotic viruses

R1 Introduction to viruses

R2 Bacteriophages

R3 DNA viruses

R4 RNA viruses

Section S-Tumor viruses and oncogenesb

S1 Oncogenes found in tumor viruses

S2 Categories of oncogenes

S3 Tumor suppressor genes

Further reading

Index